ABA, Gibberellin and Thiamine mutant stocks.

   ABA mutants. Accessions containing the genes flc, sit, and not benefit from an exogenous application of abscissic acid. A weekly foliar application should be applied from the seedling stage onward:

• 50 mg ABA (Aldrich # 86216-9) dissolved in 5 ml ethanol
• 0.4 ml Triton
• 4000 ml water

   Gibberellin mutants. Accessions containing the genes gib-1, gib-2, and gib-3 require an exogenous application of gibberellic acid. To germinate GA deficient stocks, apply a 100 micromolar concentration of gibberellic acid to seeds. In addition to the application of GA, cutting the tip of the seeds opposite the radicle increases germination rates.

   Thiamine mutants. Accessions containing the genes ten, and spa benefit from an exogenous application of thiamine. For the gene tl, exogenous applications are essential. Place vitamin B-6 pills directly in soil after transplanting or apply a foliar spray to plants throughout their life span.

References

Kerr E A. 1972. A simplified method of supplying thiamine to tomato mutants requiring it. Tomato Genetics Cooperative Report 20, 12.

Koorneef M, Bosma TDG, Hanhart CJ, Van der Veen JH, Zeevaart JAD. 1990. The isolation and characterization of gibberellin-deficient mutants in tomato. Theoretical and Applied Genetics 80, 852-857.

Sharp Robert E, LeNoble Mary E, Else Mark A, Thorne Eleanor T, Gherardi Francesca. 2000. Endogenous ABA maintains shoot growth in tomato independently of effects on plant water balance: evidence for an interaction with ethylene. Journal of Experimental Botany 51, 1575-1584.

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